Figure 4. Gene expression analysis post-MCAO.
Rats treated 10 min after MCAO with vehicle or GW3965 were sacrificed 72 h after induction of damage. Paraffin-embedded brain sections were stained with an antibody against ED1 (Serotec, Oxford UK) that detects macrophage/monocyte infiltration, and examined by light microscopy. ED1 positive cells were counted in the ipsilateral hemisphere to damage in four different coronal brain slices from 6 different animals.