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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Nature. 2010 Nov 18;468(7322):443–446. doi: 10.1038/nature09544

Fig. 1.

Fig. 1

MeCP2 silences L1 expression. a, Methylation of the L1 5’UTR-Luc reduced its transcriptional activity. b, Reduction of MeCP2 transcripts correlates with increased L1 promoter activity. c, Increased L1 promoter activity in the absence of MeCP2 but not MBD1. d, L1 RNA levels correlate with MeCP2 expression. e, Expression of the MeCP2-VP16 increased the activity of the L1 5’UTR promoter. f and g, Recruitment of MeCP2 on L1 sequences by ChIP in neural stem cells (NSC) or neurons, using 5’UTR primers (f) and two ORF2 regions (g). h, Occupancy of MeCP2 on the L1 promoter requires DNA methylation. Removal of DNA methylation with 5-Azacytidine (5-Aza) reduced MeCP2 association to L1 promoter. ChIP-qPCR shows enrichment over IgG control precipitation. All experiments show experimental triplicates. Error bars in all panels show s.e.m.