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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Neurosci. 2010 Oct 31;13(12):1463–1471. doi: 10.1038/nn.2665

Figure 4. Myosin 2B is not essential for interkinetic nuclear migration.

Figure 4

(a) Western blot showing a substantial 65-70% decrease in myosin IIB expression in Rat2 cells transfected with myosin IIB shRNA for 3 days. (b)-(e) E18 rat brain slices monitored by live time-lapse imaging following myosin II RNAi or exposure to the myosin II inhibitor blebbistatin. Nuclei were imaged using CFP-histone-H1 and either GFP for RNAi or DsRed for use with blebbistatin. GFP (green) and CFP-histone-H1 (magenta) were co-expressed with and without myosin IIB shRNA (Movies S15, S16). No apparent effect on basally directed movement of radial glial nucleus is observed relative to control (b, c). Cells co-transfected with DsRed (red) and CFP-histone-H1 (blue) were treated with 50 μm blebbistatin for 3-5 hrs during live cell imaging. No effect was observed on apically directed (d) or basally directed (e) nuclear movement in radial glial cells. However, cell in (f) was blocked in mitosis as expected. (White dashed lines indicate ventricular surface; see Movies S17-S19). Bar = 5 μm. (g) Duration of mitosis increases about 2-fold in cells subjected to myosin IIB RNAi. **: p<0.01. (H) Velocities of apically and basally directed movements in cells treated with myosin IIB shRNA and blebbistatin showing no significant difference between these groups and the control.