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. 2011 Mar 16;6(3):e17585. doi: 10.1371/journal.pone.0017585

Figure 2. Determination of Y. pestis viability using the LIVE/DEAD® BacLight™ Bacterial viability kit.

Figure 2

Panel A is a representative field of a single colony grown for 48 hours on TSA II agar at 37°C and resuspended in PBS. Panel B is a representative field of a single colony grown for 48 hours on TSA II agar at 37OC and resuspended in PBS and heat-killed for 10 minutes at 95°C. Panel C is a representative field of the microcosm after 46 days at 4°C. Cells fluorescing green are considered alive while red fluorescing cells are considered dead.