Figure 3. Autoradiogram of an SDS-PAGE gel following radiolabeled amino acid uptake by the non-culturable Y. pestis in an autoclaved tap water microcosm.
Actively growing control cells demonstrate uptake and incorporation within 24 hours whereas the cells within the nonculturable microcosm did not incorporate radiolabel until later (144 hours). The nonculturable cells incorporated amino acids into different size proteins than the actively growing control group as indicated by the arrow.