Table 3. The lytic activity, determined by the turbidimetric method, against Micrococcus lysodeikticus cells.
Concentration (mg/ml)1 | Activity (mean ± S.D., U/mg) | |
Commercial HLZ | 0.901 | 114,413.8±22,470.78 |
rHLZ | 0.1966 | 117,089.3±9,471.50 |
rHLZ-12 | 0.1202 | 79,444.44±3,367.88 |
HLZ3 | 0.04832 | 141,979±21,891.54 |
Hew-LZ4 | 86.3764 | 42,604.25±5,657.47 |
The concentration of HLZ and rHLZ was quantified by ELISA. The commercial hew-LZ was quantified by UV specrophotometer.
rHLZ-1 is purified rHLZ that was not desalinated.
HLZ is purified lysozyme from human milk that was purified using the same methodology used to purify rHLZ.
Hew-LZ is hen egg white lysozyme.