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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Cancer Res. 2010 Dec 15;70(24):10310–10320. doi: 10.1158/0008-5472.CAN-10-2062

Figure 1. Cdt1 protein level is important for MLN4924 induced re-replication in HCT116 cells.

Figure 1

(A) HCT116 cells were transfected twice with siGL2 or siCdt1 at 0- and 24-hour time point and incubated for a total of 48 hours before the addition of 0.3μM MLN4924 or DMSO. Cells were harvested for PI FACS after 20 hours of treatment. The percentage of cells containing >4N DNA was shown. (B) Total cell lysates from (A) were blotted with indicated protein antibodies. (*: non-specific band) (C) HCT116 WT or e83 cells were treated with DMSO or 0.3μM MLN4924 for 20 hours before harvested for FACS. The percentage of cells containing >4N DNA was shown. (D) Similar assay as described in (A) was performed with siMcm7. The percentage of cells containing >4N DNA was shown. (E) Similar assay as in (A) was performed with siCdc6.