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. 2011 Mar 16;6(3):e17772. doi: 10.1371/journal.pone.0017772

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Human PTH(1–34) (100 nM) or PGE₂ (5 nM–5 µM) or vehicle control (0.05% DMSO) was added to UMR 106.01 cells for 3 hours. Total RNA was collected and analyzed for Sost and Rpl32 expression by qPCR. n = 6–10 samples per treatment. a indicates p<0.05 versus Control; b indicates P<0.05 versus 5 nM PGE₂. (B) Sost mRNA expression was quantified in UMR 106.01 cells after 0, 1, 2, 3, 6, or 24 hours treatment with 5 µM PGE₂ or vehicle control. n = 4 samples per treatment. For PGE₂, each time point is significantly different (p<0.05) from Control, while for PTH, every time point except 1 hr is significantly different (p<0.05) from Control.