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. 2011 Mar 16;6(3):e17772. doi: 10.1371/journal.pone.0017772

Figure 3. PGE2 receptor expression and influence of PGE2 selective agonists upon Sost expression.

Figure 3

(A) UMR106.01 cells analyzed for Ptger1, Ptger2, Ptger3, and Ptger4 transcript expression by qPCR. Data are normalized to Rpl32. n = 4 samples. (B) UMR106.01 cells were cultured with DMSO as vehicle control, 100 nM hPTH(1–34), 5 µM PGE2 in the presence and absence of inhibitors of protein kinase A (H-89, 2.5 µM) or phospholipase C (U73122, 10 µM), for 3 hours. Total RNA was analyzed for Sost and normalized to Rpl32. n = 4–8 samples. Compared to solvent control, a indicates p<.001 and b indicates p<0.05; c indicates p<.001. (C) UMR106.01 cells were cultured with DMSO as vehicle control, 100 nM hPTH(1–34), the cell-permeant cyclic AMP analogue 8-br-cAMP (1 mM) or the calcium ionophore ionomycin (1.3 µM) for 3 hours, after which total RNA was collected and analyzed for Sost and Rpl32. n = 4–8 samples. Versus Control, a indicates p<0.05, b indicates p<0.01, and c indicates p<0.001.