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Composition of basic (PDM) and optimised (PDM-pcDNA3F) medium for DH5α-pcDNA3F cultivation.
| Nutrient | PDM (g/L) |
PDM-pcDNA3F (g/L) |
|---|---|---|
| yeast extract | 4.41 | 4.41 |
| tryptone | 7.93 | 10.60 |
| glucose | 10.00 | 6.50 |
| Na2HPO4 | 12.80 | 15.70 |
| K2HPO4 | 3.00 | 3.00 |
| NH4Cl | 0.50 | 0.50 |
| MgSO4 | 0.24 | 0.24 |
