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. Author manuscript; available in PMC: 2011 May 17.
Published in final edited form as: Cell Cycle. 2010 Mar 15;9(6):1156–1166. doi: 10.4161/cc.9.6.11049

Figure 5.

Figure 5

Dna2 and double-strand breaks. (A) Dna2 interacts with DSB proteins. Anti-Dna2 antibodies were used to immunoprecipitate Dna2 from interphase extract, and immunblots were performed for Dna2, ATM and Nbs1. (B) Dna2 accumulates on damaged chromatin. Sperm chromatin was added to interphase extracts in the presence or absence of 0.1 units/μl PflM1 and/or chekpoint inhibitors (5 mM caffeine or 0.1 mM wortmannin) as indicated. After a 100 min. incubation, chromatin fractions were isolated from extract as previously described30 and protein levels on chromatin were analyzed by immunoblotting.