Skip to main content
. Author manuscript; available in PMC: 2012 Mar 22.
Published in final edited form as: Biochemistry. 2011 Feb 15;50(11):1831–1838. doi: 10.1021/bi1016777

Fig. 5.

Fig. 5

A. ADP release from actomyo1c3IQ. The fractional amplitudes of the slow phase were obtained by fitting pyrene-actomyo1c dissociation transients from experiments with (circles) myo1c3IQ or (squares) R156W-myo1c3IQ to a double exponential as a function of ADP concentration at 37 °C. Values were normalized to the total change in amplitude. The lines are fits of the (solid) myo1c3IQ and (dashed) R156W-myo1c3IQ data to a hyperbolic function. The inset shows time courses of pyrene-actin fluorescence after mixing 500 µM ATP with 0.25 µM myo1c3IQ-pyrene-actin and R156W-myo1c3IQ-pyrene-actin equilibrated with 10 µM ADP. The smooth lines are best fits of the data to a single exponential function. B. Calculated duty ratio as a function of actin concentration for (solid) myo1c3IQ and (dashed) R156W-myo1c3IQ. Duty ratios were calculated as defined in equation 2.