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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochem Pharmacol. 2011 Jan 15;81(7):910–916. doi: 10.1016/j.bcp.2011.01.005

Figure 3.

Figure 3

Activation of ERK1/2 by EGF protects Huh7 cells from fenretinide-induced apoptosis. Cells were seeded onto a 96-well plate and treated with fenretinide (10 μM) or EGF (0.2 μg/ml) for 24 hrs. For the combination treatment, Huh7 cells were treated with EGF for 2 hrs followed by EGF and fenretinide combination treatment for additional 24 hrs. Caspase 3/7 activity and cell survival were determined by Caspase-Glo® 3/7 and CellTiter-Glo® Luminescent Cell Viability Assay (Promega), respectively as described in the Materials and Methods. Data were expressed as mean ± SD from three independent experiments, **p<0.01, vs. DMSO; #p<0.05.