Figure 6.

The expression levels of Bcl-2, Bcl-xL, Bax, and Bid are not associated with the apoptotic effect induced by fenretinide, PD98059, or EGF treatments. HepG2 (A) and Huh7 (B) cells were treated as described in figure legends 2 and 3, respectively. Proteins were extracted for western blot using specific antibodies as described in the Materials and Methods. GAPDH level was used for loading control. Representative data are shown from three independent experiments.