Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: Anal Chem. 2011 Jan 5;83(3):774–781. doi: 10.1021/ac102975g

Fig.5 — Cell velocity comparison between two different inlet devices. a) Cell velocity comparison. Cell velocity at vertical inlet area was smaller than in the channel (0.07±0.03 mm/s vs. 0.48±0.08 mm/s). There was no significant difference (compared by t test) for the parallel inlet device (0.46±0.06 mm/s vs. 0.47±0.05 mm/s). Error bars represent standard deviation of cell velocity. b) Typical cell velocity changes over time in two devices. Cell velocity was tracked frame by frame. The cell in the parallel inlet device moved out of inlet area between 0.22 and 0.33 second, while the cell in the vertical inlet device moved out of the inlet area between 0.33 and 0.44 second. The velocity of the cell in the vertical inlet increased after moving out of the inlet area. Cell velocity was measured from videos recorded during the experiments at camera speed 9 frame/s. Videos were analyzed by imageJ to measure cells displacement in pixels between each frame. 1 pixel in the videos represents 0.001288 mm in actual scale.

Fig.5 — Cell velocity comparison between two different inlet devices. a) Cell velocity comparison. Cell velocity at vertical inlet area was smaller than in the channel (0.07±0.03 mm/s vs. 0.48±0.08 mm/s). There was no significant difference (compared by t test) for the parallel inlet device (0.46±0.06 mm/s vs. 0.47±0.05 mm/s). Error bars represent standard deviation of cell velocity. b) Typical cell velocity changes over time in two devices. Cell velocity was tracked frame by frame. The cell in the parallel inlet device moved out of inlet area between 0.22 and 0.33 second, while the cell in the vertical inlet device moved out of the inlet area between 0.33 and 0.44 second. The velocity of the cell in the vertical inlet increased after moving out of the inlet area. Cell velocity was measured from videos recorded during the experiments at camera speed 9 frame/s. Videos were analyzed by imageJ to measure cells displacement in pixels between each frame. 1 pixel in the videos represents 0.001288 mm in actual scale.