Table 2.
Kinetic parameters and inhibition constants of AgAK
| Km (nM) | kcat (s−1) | kcat/Km (M−1 s−1) | Ki (nM) | |
|---|---|---|---|---|
| Adenosinea | ||||
| ATP | 8.1 ± 0.6 | 0.160 ± 0.003 | 1.97 ± 0.04 × 107 | - |
| GTP | 230 ± 10 | 0.63 ± 0.01 | 2.7 ± 0.1 × 106 | - |
| UTP | 740 ± 50 | 0.035 ± 0.002 | 4.7 ± 0.4 × 106 | - |
| CTP | 630 ± 50 | 0.029 ± 0.001 | 4.6 ± 0.4 × 106 | - |
| TTP | 790 ± 80 | 0.056 ± 0.004 | 7.1 ± 0.9 × 106 | - |
| NTPb | Km (μM) | |||
| ATP | 1.4 ± 0.4 | 0.33 ± 0.02 | 2.4 ± 0.5 × 105 | - |
| GTP | 54 ± 5 | 1.39 ± 0.07 | 2.6 ± 0.3 × 104 | - |
| UTP | 56 ± 2 | 0.061 ± 0.001 | 1.09 ± 0.04 × 103 | - |
| CTP | 49 ± 3 | 0.062 ± 0.002 | 1.27 ± 0.09 × 103 | - |
| TTP | 245 ± 74 | 0.30 ± 0.07 | 1.2 ± 0.5 × 103 | - |
| ITU | - | - | - | 1.0 ± 0.2 |
| Ap4A | - | - | - | 860 ± 130 |
| Ap3A | - | - | - | 61,000 ± 13,000 |
a
Km values for adenosine were determined at fixed concentrations of 100 μM of each NTP with other conditions as described in the methods.
b
Km values for the NTPs were determined at fixed concentrations of 10 μM adenosine with other conditions as described in the methods.