Brain sections from 20-wk 9H/PS-NA mice were examined by dual antibody staining. (Upper panels) Cortical regions stained with monoclonal anti-mouse GFAP for astrocyte (Alexa-488, green)/polyclonal anti-mouse α-synuclein (Alexa-610, red). (Lower panels) Hippocampal CA2 regions stained with anti-CD68 for microglial cell (Alexa-488, green)/α-synuclein (Alexa-610, red). The GFAP signals did not colocalized with α-synuclein signals. α-Synuclein signals were not merged with CD68 signals, but some were within microglial cells (arrows). The scale bars are 20 μm. No significant α-synuclein, GFAP and CD68 signals were present in WT brain sections (not shown).