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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Genet Metab. 2010 Dec 31;102(4):436–447. doi: 10.1016/j.ymgme.2010.12.014

Figure 7. Aggregated cytoplasmic proteins in the brain neurons from 9H/PS-NA mice.

Figure 7

Serial brain sections from 23-wk 9H/PS-NA mice were processed for silver and α-synuclein and ubquitin immunofluorescence staining. Silver staining images show the presence of dense dark particles in cerebellar white matter of 9H/PS-NA mouse (A), but not in heterozygote littermate controls (D). The adjacent sections were processed for immunofluorescence staining using anti α-synuclein (Alexa-610, red) and anti ubiquitin (Alexa-488, green) antibodies. Arrows indicate the α-synuclein positive aggregates (B) or ubiquitin signals (C) in 9H/PS-NA sections. Yellow arrows indicate appositionally matched silver aggregates with both α-synuclein (B) and ubiquitin (C) Red arrows indicate the silver signals (A) that matched only with α-synuclein (B). The green arrow indicates that the silver signals in (A) matched only with ubiquitin signals (C). Brain tissues from heterozygote littermates were processed in parallel and no α-synuclein (E) or ubiquitin particles (F) were observed. The scale bars are 50 μm.