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BPI chromatogram for the LC-MS analysis of 200 fmol of a tryptic digest of a BSA and enolase mixture. LC flow rate: 65 nL/min, MPA: 0.1% formic acid in water, MPB: 0.1% formic acid in acetonitrile, gradient: 5 to 50% B in 30 min, flow rate: 65 nL/min.
