PMT cells that project to the contralateral IC and the ipsilateral MG. For each column, the top panel represents labeling from a GB injection in the right MG, the middle panel represents labeling from a RB injection in the left IC, and the bottom panel represents immunolabeling for ChAT. All three panels show the same field of view using different filters to visualize the different fluorescent labels. Cells in the right PPT (A) and LDT (B) labeled for GB (top panel), RB (middle panel), and ChAT immunoreactivity. Arrowheads show cells that project to the contralateral IC and ipsilateral MG and are ChAT immunopositive. GP 484. B) Cell in the right LDT that projects to the contralateral IC and ipsilateral MG and is ChAT immunopositive. GP 484. Scale bars = 10 μm. cIC, contralateral IC; cMG, contralateral MG; iIC, ipsilateral IC; iMG, ipsilateral MG.