Table 1.
Summary of injection sites and immunolabel. List of tracers injected in each case and the fluorescent label used to visualize the ChAT immunoreactivity. Values in parentheses indicate the total volume of tracer injected into the indicated structure. “x” indicates that no injections were made at that location in that animal. * denotes a Nanoliter injection; all other injections were made with a microsyringe. AF 488 – AlexaFluor 488 [green]; AF 647 – AlexaFluor 647 [near-infrared]; FB – Fast Blue (EMS-Chemi GmbH, Gross Umstadt, Germany); FG -FluoroGold (FluoroChrome, Inc., Englewood, CO, USA); GB - green beads (Luma-Fluor, Inc., Naples, FL, USA), RB – red beads (Luma-Fluor, Inc.).
| Case | Tracer in left IC (total volume) | Tracer in left MG (total volume) | Tracer in right MG (total volume) | Immunolabel |
|---|---|---|---|---|
| GP 481 | RB (0.6 μl) | FG (0.05 μl) | GB (0.4 μl) | AF 647 |
| GP 482 | RB (0.6 μl) | FG (0.05 μl) | GB (0.4 μl) | AF 647 |
| GP 484 | RB (0.6 μl) | FG (0.05 μl) | GB (0.2 μl) | AF 647 |
| GP 585 | FB (0.6 μl) | GB* (69 nl) | RB* (69 nl) | AF 647 |
| GP 586 | FB (0.6 μl) | GB* (69 nl) | RB* (69 nl) | AF 647 |
| GP 587 | FB (0.6 μl) | GB* (69 nl) | RB* (69 nl) | AF 647 |
| GP 595 | FG (0.3 μl) | GB* (69 nl) | RB* (69 nl) | AF 647 |
| GP 604 | GB (0.6 μl) | FG (0.05 μl) | x | AF 647 |
| GP 633 | x | RB (0.4 μl) | FG (0.05 μl) | AF 488 |