Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Cancer Res. 2011 Jan 19;71(6):2108–2117. doi: 10.1158/0008-5472.CAN-10-1998

Figure 1.

Figure 1

Efficient and stable synthesis of alternatively spliced AR mRNA isoforms in CRPCa cells. A, Growth of CWR22Pc and 22Rv1 cells in the presence or absence of androgens. B, Plasmid templates harboring depicted cDNAs were subjected to PCR with indicated primer pairs. Right panels, mRNA from CWR22Pc and 22Rv1 cells was subjected to quantitative RT-PCR using indicated primer sets. Ct values obtained from qRT-PCR reactions were converted to copy number by plotting sample Ct values on Ct vs. copy number standard curves constructed from concurrent qPCR analysis of serial dilutions of plasmid templates. Data represent Mean +/- Standard Error from two independent experiments, each performed in triplicate (n = 6). C, AR Western blots of CWR22Pc and 22Rv1 cells following 3 day treatment with 1nM DHT. ERK-2, loading control. D, AR Western blot of 22Rv1 cells following 10 day culture in steroid depleted medium containing 1nM DHT or vehicle control. ERK-2, loading control.