Figure 1. JPH2 expression is decreased in humans with hypertrophic cardiomyopathy.
A. Representative Western blot of JPH2 expression levels in lysates prepared from left ventricular myocardium obtained from three healthy traumatic death victims and myectomy tissue from 11 patients with HCM genotype-positive for mutations in MYH7 (N = 5 individuals), MYBPC3 (3) or genotype-negative (3). From left to right, HCM patients hosted mutations of MYH7-G741R, -R663H, -I736T, -R403Q, -M922K and MYBPC-E258K, -L527 fs/3, and -1258 fs/71, respectively. GAPDH served as a loading control. B. Graph of relative mean band density of JPH2 expression of individuals with HCM (mean of 8 blots per individual) relative to control specimens (mean of 5 blots per individual). ‡, P < 0.05 versus each of the trauma samples; †, P < 0.05 versus two of the three trauma samples. Inset) Graph of the mean Western blot band density of all HCM specimens relative to mean control tissue band density. ***, P < 0.0001.