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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Circ Heart Fail. 2011 Jan 7;4(2):214–223. doi: 10.1161/CIRCHEARTFAILURE.110.958694

Figure 4. JPH2 knock-down reduces intracellular Ca2+ transients.

Figure 4

A. Representative Ca2+ transient tracing of an HL-1 cell transiently transfected with pCMS-shJPH2 or shLuc measuring relative fluorescent intensity (Fmax/Fo) versus time. B. Bar graph of the mean Ca2+ transient amplitude in HL-1 cells transiently transfected with pCMS-shJPH2 or control. Cells were treated with LTCC activators BayK or FPL or LTCC blocker nifedipine (Nif). Numbers in bar graphs indicate number of cells analyzed. **, P < 0.01; ***, P < 0.0001 C, and D. Bar graph of time to transient peak, and time to decay to half maximum intensity, respectively, in HL-1 cells transiently transfected with pCMS-shJPH2 or control. ***, P < 0.0001 E. Bar graph of the mean resting Ca2+ levels in cells transiently transfected with pCMS-shJPH2 or control. Numbers in bar graphs indicate number of cells analyzed.

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