Alignment of nine LOV-domain sequences and identification of
flavin-interacting residues including LOV1 and LOV2 domains from
Adiantum phy3 (GenBank accession no. BAA36192),
Arabidopsis phototropin (GenBank accession no.
AAC01753), oat phototropin (GenBank accession no. AAC05083),
Arabidopsis ztl (GenBank accession no. AAF70288),
Arabidopsis fkf1 (GenBank accession no. AAF32298), and
Neurospora Wc-1 (GenBank accession no. Q01371). LOV2
residues that interact with FMN are marked with vertical arrows.
Secondary structure of LOV2 is marked above alignment: β-strand,
arrows; helix, bars. Green residues are conserved in all LOV1 and LOV2
domains included in the alignment as well as eight other LOV domains
(not included because of space limitations) from rice phototropin
(BAA84779), corn phototropin (T01353), rice phototropin-like protein
nph1b (GenBank accession no. BAA84779) and Arabidopsis
phototropin-like protein npl1 (GenBank accession no. AAC27293). Blue
residues are conserved in all LOV1 domains, and red residues are
conserved in all LOV2 domains from the above-listed proteins. This
color scheme is applied to residues in Wc-1, ztl, and fkf1.