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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Clin Cancer Res. 2010 Dec 15;16(24):6060–6070. doi: 10.1158/1078-0432.CCR-10-1624

Figure 4.

Figure 4

Co-treatment inhibits GBM growth and colony forming ability. A, Sample wells from anchorage independent growth assay where HSR-GBM1 cells were treated with vehicle, 2 μM MRK-003, 5 μM cyclopamine, or both drugs. B, upper graph, Growth of HSR-GBM1 in soft agar with treatment, repeated in triplicate; lower graph HSR-GBM1 pre-treated for seven days before plating in soft agar and then grown without drug. C, HSR-GBM2 and U87 grown in soft agar with drug treatment. Performed in triplicate for N=3 for all cell lines. * P<0.02, ** P<0.001, *** P<0.0001, using Student’s t-test.