TABLE 2.
Dots (.) indicate no difference to the Sylvester sequence, a base call (according to IUPAC recommendations) shows the substitution at a distinct position, Nucleotide substitutions: R=A/G (Purine), Y=C/T (Pyrimidine), W=A/T, denoted with the symbol – Δ.
Nucleotide position counted from origin (33) at top, in reference to amplified sequence at bottom. Insertions denoted as the position number, a decimal point and the number of nucleotides inserted.
Consensus “Sylvester” reference sequence generated to produce the least number of variants to describe all other mitotypes.