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ICMs were isolated from mouse blastocysts. (A) Fresh ICM before vitrification. (B-E) Morphology of vitrified—warmed ICMs after plating on MEF feeder layer. (B) 3 hours after warming (C) 24 hours (D) 48 hours (E) 72 hours. (F) Dome-like ICM outgrowth staining positive for alkaline phosphatase. Trophectodermal and MEF cells adjacent to ICM were negative for AP
