TABLE 1.
Proteinb | Accession numberc | M.W. (kDa) | Quantity (pmol)a |
Ratio (B/A) | |
---|---|---|---|---|---|
A | B | ||||
Myoglobin | 161 | 16.5 | 0.50 | 5.00 | 10.00 |
Ubiquitin | 4014 | 8.7 | 5.00 | 23.00 | 4.60 |
Cytochrome c | 3870 | 13.0 | 2.50 | 11.50 | 4.60 |
HRP | 2466 | 43.3 | 5.00 | 11.00 | 2.20 |
Serum albumin | 1213 | 66.6 | 5.00 | 3.33 | 0.67 |
Catalase | 465 | 57.5 | 0.50 | 0.34 | 0.67 |
Carbonic anhydrase I | 69 | 28.9 | 2.50 | 1.14 | 0.45 |
Lactoperoxidase | 2648 | 77.5 | 2.50 | 0.78 | 0.31 |
Glucose oxidase | 152 | 80.0 | 0.50 | 0.33 | 0.67 |
Glycerokinased | 904 | 54.0 | 2.50 | 0.78 | 0.31 |
Hexokinase | 2938 | 50.0 | 0.50 | 0.16 | 0.31 |
Tryptophanased | 2366 | 51.0 | 5.00 | 1.56 | 0.31 |
aSample C contained the same quantities of protein as sample B.
bProteins were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
cAccession number in PRG database.
dAdded E. coli proteins.