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. Author manuscript; available in PMC: 2011 Mar 17.
Published in final edited form as: Nature. 2010 Jul 11;466(7305):508–512. doi: 10.1038/nature09272

Figure 3. PHF8 regulates E2F target genes in conjunction with HCF-1 and Set1A during G1/S transition.

Figure 3

a, Venn diagram showing overlapping between PHF8 ChIP-Seq. peaks in G1 and G1/S phases (n= 9,211 and 20,846, respectively). b–d, ChIP analysis of PHF8 (b), H4K20me1 (c) and L3MBTL1(d) on selected promoter regions in HeLa cells synchronized to G1 or G1/S phases. e, Cell extracts from Flag-PHF8 and GFP-HCF-1 co-transfected HEK293T cells were immunoprecipitated and analyzed by immunoblotting as indicated. f, Cell extracts from Flag-PHF8 and HA-HCF-1 (N1011) (amino-terminus of HCF-1) or HA-HCF-1 (C600) (Carboxyl-terminus of HCF-1) co-transfected HEK293T cells were immunoprecipitated and analyzed by immunoblotting as indicated. g–h, ChIP analysis of HCF-1 (g) or Set1A (h) on selected promoter regions in HeLa cells transfected with control, PHF8 or HCF-1 siRNAs. (± s.e.m., *p<0.05, **p<0.01, ***p<0.001)

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