Figure 2.

Typical force-extension curves of G6-53 at different pH measured at a pulling speed of 400 nm/s. Stretching polyproteins of (G6-53)₈ results in characteristic sawtooth-like force-extension curve, in which the individual force peaks correspond to the mechanical unfolding of the individual domain. The last peak corresponds to the detachment of the unfolded polyprotein chain from either the AFM tip or substrate. Gray lines correspond to the WLC fits to the experimental data. On decreasing the pH, the mechanical unfolding force of G6-53 showed significant decrease.