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Unfolding force of G6-53 does not change with pH at ionic strength of 2 M. The average unfolding forces of G6-53 are 104 ± 32 pN at pH 8.5(n = 266), 111 ± 34 pN at pH 7 (n = 431), 112 ± 28 pN at pH 6 (n =166), 115 ± 28 pN at pH 5 (n = 131), 107 ± 31 pN at pH 4 (n = 375), respectively. At higher ionic strength, the electrostatic interactions between the two histidine residues are screened and the mechanical unfolding force of G6-53 no longer shows pH-dependence.
