Figure 1.

A schematic representation of the sciatic nerve crush model used to assess axon remyelination and regeneration. Tamoxifen (Tamox.) or vehicle (Veh.) was administered to animals of 10 weeks of age for 5 d. At 4 weeks later, the sciatic nerve was crushed at the midthigh level. At 10 d, 14 d, or 8 weeks after sciatic nerve crush, this nerve was harvested at a point 2 mm distal to the crush site and processed for immunoelectron microscopy. A whole-mount preparation of the tibial nerve was developed to visualize regenerating YFP-positive axons. The gastrocnemius muscle (which is innervated by the tibial nerve) was used for studies of neuromuscular junction reinnervation.