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. Author manuscript; available in PMC: 2011 Mar 17.
Published in final edited form as: Cell. 2010 Sep 17;142(6):902–913. doi: 10.1016/j.cell.2010.08.029

Figure 6. M2 Localizes to the Neck of Budding Virions.

Figure 6

MDCK cells were infected with an MOI of 3 pfu/cell of A/Udorn/72 for 18 h. (A) Virus-infected cells were fixed and processed for immunofluorescent detection of HA (shown in green) and M2 (shown in red). (B) Magnification of images shown in Fig. 6a. Scale bars indicate 10 μm. (C) Virus-infected cells were stained for M2 via immuno-gold labeling and thin sections were analyzed by electron microscopy. (D) Magnification of images shown in Fig. 6c. Scale bars indicate 100 nm. Arrows indicate M2 foci at sites of virus budding. See also Fig. S6.