Figure 1.

Far Western blot with specific granules from normal or CGD patient overlaid with labeled p67^PHOX. Specific granules (2.5 × 10⁷ cell equivalents) from a normal subject or a CGD patient were resolved by 12% SDS/PAGE and blotted onto nitrocellulose. The blot then was incubated with p67^PHOX labeled by phosphorylation with [γ-³²P]ATP. After washing five times, the blot was exposed to x-ray film (Left). The same blot then was stripped as described in Experimental Procedures and incubated with a mAb against gp91^PHOX (Right).