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Binding of p67PHOX to immobilized cytochrome b558. Eight and six-tenths pmol each of pure cytochrome b558, p47PHOX (positive control), carbonic anhydrase, or BSA was applied directly to nitrocellulose filters, which were incubated with p67PHOX (1.5 μg/ml) as described in Experimental Procedures. The filters then were washed and incubated with anti-p67PHOX antibody. (Top) Native proteins. (Middle) Denatured proteins. (Bottom) No p67PHOX.
