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. 2001 Mar 13;98(6):3001–3005. doi: 10.1073/pnas.061029698

Figure 4.

Figure 4

Effect of Rac1 and Cdc42 on the binding of p67PHOX to cytochrome b558. The experiments were performed as described in the text, except that 800 pmol of Rac-GTP[γS]/GDP[βS] or Cdc42-GTP[γS]/GDP[βS] was used. (A Upper) Affinity precipitation in the presence of Rac. Lanes: 2, plus 800 pmol Rac1; 3, plus 800 pmol Rac1-GTP[γS]; 4, plus 800 pmol Rac1-GDP[βS]. (A Lower) Quantification of the binding by scanning densitometry. The data are expressed as percentage of full-length GST-p67PHOX and are the mean ± SE of three experiments. (B Upper) Affinity precipitation in the presence of Cdc42. Lanes: 2, plus 800 pmol Cdc42; 3, plus 800 pmol Cdc42-GTP[γS]; 4, plus 800 pmol Cdc42-GDP[βS]. (B Lower) Quantification of the binding by scanning densitometry. The data are expressed as percentage of full-length GST-p67PHOX and are the mean ± range of two experiments.