Table 2.
Equilibrium constant and Gibbs free-energy change values for TMPyP4 binding to the WT, 1:6:1 mutant, and 1:2:1 mutant 24-mer c-MYC promoter quadruplex sequences at 30 mM [K+] and 130 mM [K+] salt concentrations
| 30 mM [K+] salt concentrations | ||||||
|---|---|---|---|---|---|---|
| 30 mM [K+] BPES | K1 (M−1) × 10−6 | ΔG1 | K2 (M−1) × 10−6 | ΔG2 | K3 (M−1) × 10−6 | ΔG3 |
| WT | 82 ± 1 | −10.6 | 3.0 ± 0.5 | −8.8 | – | – |
| 1-6-1 | 2020 ± 3 | −12.7 | 30.4 ± 1 | −10.2 | 1.3 ± 0.5 | −8.3 |
| 1-2-1 | 8320 ± 9 | −13.5 | 31.2 ± 1 | −10.2 | 1.9 ± 0.8 | −8.6 |
| 130 mM [K+] salt concentrations | ||||||
|---|---|---|---|---|---|---|
| 130 mM [K+] BPES | K1 (M−1) × 10−6 | ΔG1 | K2 (M−1) × 10−6 | ΔG2 | K3 (M−1) × 10−6 | ΔG3 |
| WT | 5 ± 0.5 | −9.1 | 0.2 ± 0.1 | −7.0 | – | – |
| 1-6-1 | 115 ± 3 | −11.3 | 10.8 ± 0.8 | −9.6 | 0.50 ± 0.08 | −7.6 |
| 1-2-1 | 86 ± 1 | −10.8 | 0.5 ± 0.1 | −7.7 | – | – |