Table 3.
ITC-derived thermodynamic parameters for TMPyP4 binding to the WT, 1:6:1 mutant, and 1:2:1 mutant 24-mer c-MYC promoter quadruplex sequences
| 30 mM [K+] salt concentration | ||||||
|---|---|---|---|---|---|---|
| 30 mM [K+] BPES | ΔH1 (kcal/mol) | −TΔS1 (kcal/mol) | ΔH2 (kcal/mol) | −TΔS2 (kcal/mol) | ΔH3 (kcal/mol) | −TΔS3 (kcal/mol) |
| WT | −1.7 (±0.1) | −9.0 | −11.2 (±0.2) | 2.3 | – | – |
| 1-6-1 | −6.0 (±0.2) | −6.6 | −11.4 (±0.8) | 1.2 | −16.8 (±1.3) | 8.4 |
| 1-2-1 | −6.0 (±0.1) | −7.5 | −10.1 (±0.8) | −2.8 | −16.3 (±0.6) | 7.7 |
| 130 mM [K+] salt concentration | ||||||
| 130 mM [K+] BPES | ΔH1 (kcal/mol) | −TΔS1 (kcal/mol) | ΔH2 (kcal/mol) | −TΔS2 (kcal/mol) | ΔH3 (kcal/mol) | −TΔS3 (kcal/mol) |
| WT | −0.7 (±0.1) | −8.4 | −8.2 (±1.7) | 1.3 | – | – |
| 1-6-1 | −3.5 (±0.2) | −8.0 | −8.6 (±1.7) | −1.0 | −5.0 (±0.9) | −2.7 |
| 1-2-1 | −3.5 (±0.2) | −7.3 | −7.6 (±0.4) | −0.2 | – | – |