Skip to main content
. Author manuscript; available in PMC: 2011 Mar 17.
Published in final edited form as: J Am Chem Soc. 2010 Jun 16;132(23):8010–8019. doi: 10.1021/ja1003922

Figure 1.

Figure 1

Comparison of two-dimensional, double mutant vs. semi-mutant cycles. (A) The classic double mutant cycle construct (with respect to global unflolding) is shown for the V34L/P58Y cycle. When this strategy is implemented using HX, this construct yields SSO couplings. (B) An HX-based semi-mutant cycle is shown in which a mutation (V34L) provides one structural perturbation and structural opening of the amide of N57 provides the other. The semi-mutant coupling results from ΔΔG because the vertical transitions are directly measured, in contrast to the classic double mutant cycle. Asterisks represent an open-closed equilibrium at a particular residue I, which may or may not be a local event. Black dots represent measured transitions in both methods.