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. Author manuscript; available in PMC: 2011 Mar 17.
Published in final edited form as: J Am Chem Soc. 2010 Jun 16;132(23):8010–8019. doi: 10.1021/ja1003922

Figure 2.

Figure 2

Site-specifically observed (SSO) coupling values, Δ2GHX, kij, for three double mutant cycles in eglin c. ΔGHX values from each of the cycle variants were used to compute SSO coupling values for each reporter via eq. 2. The first bar in each plot denotes the globally-measured value from fluorescence, while the second bar denotes the average HX-determined value from residues whose value exceeds 2σ. Red bars highlight residues that exchange via the global exchange mechanism in WT, and pink bars highlight residues that exchange via a mixed mechanism.