Figure 4. FAHF-2 inhibited mast cell proliferation and degranulation.

A. Mast cell proliferation. MC/9 cells were cultured and viable cells were counted at indicated time points. Cell numbers are expressed as fold increase vs day 0. Data are shown as Means ± SEM of 3 individual experiments. * p<0.05, ** p<0.01 vs. IgE alone. ##, p<0.01 vs Medium alone (Med). B. Mast cell degranulation. MC/9 cells were cultured and then challenged at different conditions as indicated. Supernatant and total cell histamine levels were determined. Data expressed as percent of histamine release (Means ± SEM of 3 individual experiments). ***p<0.001vs IgE + DNP without FAHF-2 (1^st bar).