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Detection of potential CCT–gelsolin interactions in T47D cells using a proximity ligation assay. Proximity ligation assays were performed using primary antibody pairs against CCTε/gelsolin and CCTε/γ-catenin, both with anti-rabbit plus and anti-mouse minus PLA probes. Controls with one primary antibody and both PLA probes are indicated (a). The scale bar represents 20 μm. PLA signals/cell were counted from five fields of view (b). Error bars represent the standard error where n = 5
