Fig. 2.

Artemin synthesis and purification. Protein extracts from transformed bacteria were resolved in SDS polyacrylamide gels and either stained with Coomassie blue (a) or blotted to nitrocellulose and reacted with antibody to artemin (b). Lanes 1 and 2 ArtWT, 3 and 4 ArtC22A, 5 and 6 ArtC61A, 7 and 8 ArtC166A, 9 and 10 ArtC172A. Samples in odd- and even-numbered lanes were respectively from non-induced and induced bacteria. All lanes received equal amounts of protein. Artemin purified by affinity chromatography was electrophoresed in SDS polyacrylamide gels and either stained with Coomassie blue (c) or blotted to nitrocellulose and reacted with antibody to artemin (d). All lanes received equal amounts of protein. Arrowhead artemin. M molecular mass marker (kDa)