Artemin chaperone activity in vitro. Artemin was heated at 43°C for 1 h with 150 nM citrate synthase, and solution turbidity (absorbance) was measured at 360 nm. The final artemin concentrations, based on monomer molecular mass, were: 1 0 nM, 2 150 nM, 3 300 nM, 4 450 nM, and 5 600 nM. The same artemin concentrations in different panels are represented by identical symbols