Fig. 4.

Artemin chaperone activity is concentration-dependent and reduced by cysteine modification. Artemin at 600 nM (a), 450 nM (b), 300 nM (c), and 150 nM (d) was heated for 60 min at 43°C with 150 nM citrate synthase, and solution turbidity was measured at 360 nm. Artemin molarities were based on monomer molecular mass. The chaperone activity of each artemin variant, measured as the ability to inhibit turbidity development (Inhibition%), was calculated as described in “Materials and methods”