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Artemin oligomer formation is modified by mutagenesis. Bacterial protein extracts containing similar amounts of artemin were fractionated in Sepharose 6B columns. Fifteen-microliter samples from selected fractions were resolved in SDS polyacrylamide gels, blotted to nitrocellulose, and reacted with antibody to artemin followed by HRP-conjugated goat anti-rabbit IgG. Numbered arrows molecular mass markers
