Table 1.

Primers employed for the generation of nucleotide substitutions in artemin cDNA

Artemin variant	Primer sequence
ArtC22A	5′-GGCAAAGTTCAAATGGACGCCCCAAGCAGGCACAATT-3′
	3′-AATTGTGCCTGCTTGGGGCGTCCATTTGAACTTTGCC-3′
ArtC61A	5′-TTCTATGCCAGAGACGCCAAGGCTGCCGTGG-3′
	5′-CCACGGCAGCCTTGGCGTCTCTGGCATAGAA-3′
ArtC166A	5′-GGAATAAATTCCTATCCGACGCCTTGTCCAACCTACACTG-3′
	5′-CAGTGTAGGTTGGACAAGGCGTCGGATAGGAATTTATTCC-3′
ArtC172A	5′-GTCCAACCTACACGCCATCGGTTCACAAGG-3′
	5′-CCTTGTGAACCGATGGCGTGTAGGTTGGAC-3′

Single-nucleotide substitutions of artemin cDNA were generated by site-directed mutagenesis using the listed primers with modified nucleotides in bold. Substitutions were named by starting with the mutated amino acid residue, ollowed by its position, and then the replacement residue