Table 2.
Methods used by each laboratory in the study
| Lab | Polymerase | Thermocycler | Platform | Fragment labelling | Size standard | Fragment analysis |
|---|---|---|---|---|---|---|
| Lab A | Promega Go-Taq | Hybaid | Licor 4300 | IRD700/IRD800 | Made in house | By eye |
| Lab B | Bioline Biotaq | ThermoHybaid MBS system | MegaBACE500 | FAM, HEX NED | GE Healthcare-Et-ROX400 & Et-ROX550 | Fragment Profiler |
| Lab C | Qiagen hotstart; Thermostart | ABI9700, VWR Quattro, MJ Research | ABI3130 | FAM, HEX, NED, VIC, TET | GS LIZ 500 | Genemapper 3.7 |
| Lab D | QIAGEN multiplex PCR kit | ABI 2730, Eppendorf, PTC-100 | ABI 3130xl | FAM, PET, VIC, NED | GS LIZ 600 | Genemapper 3.7 |
| Lab E | GoTaq Promega; Flexi DNA polymerase | ABI 2720 | ABI 3130 | FAM, PET, VIC, NED | GS LIZ 500 | Genemapper |
| Lab F | Bioline BioTaq RED; Sigma REDTaq | ThermoHybaid | Beckman-Coulter CEQ8000 | Sigma-Genosys WellRED | GenomeLab size-standard 400 | CEQ 8000 Genetic analysis system |
| Lab G | TEG polymerase (Prokaria manufactured) | MJ-research PTC-225 | ABI-3730 | FAM, PET, VIC, NED | GS LIZ 500 | Genemapper 4.0 |
| Lab H | Promega Go-Taq | ABI9700 | ABI-3730 | FAM, PET, VIC, NED | GS LIZ 500 | Genemapper 4.0 |
| Lab I | Invitrogen Taq | Hybaid PCR Express | ABI 3130 | FAM, PET, VIC, NED | GS LIZ 500 | Genescan 4.0 |
| Lab J | QIAGEN multiplex PCR kit | AB 2720 | ABI3130xl | FAM, PET, VIC, NED | GS LIZ 500 | Genemaker |
| Lab K | QIAGEN multiplex PCR kit | ABI 2720 | ABI 3130XL | FAM, HEX, NED | ROX 500 | Genotyper 3.5 |
| Lab L | Qiagen hotstart mastermix kit | MBS ThermoHybaid | ABI 3130 | FAM, PET, VIC, NED | GS LIZ 500 | Genescan 4.0 |