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. 2011 Mar 15;25(6):594–607. doi: 10.1101/gad.2008511

Figure 1.

Figure 1.

Kdm1a mutant ES cells have normal levels of DNMT1 and DNA methylation. (A) Immunoblot analysis using the indicated antibodies was performed on Kdm1a GT/GT (lanes 1–4), +/GT (lanes 6,8), or +/+ (lanes 5,7) ES cells. (B) Immunoblot analysis of Kdm1a FL/FL Cre-ERT ES cells treated with vehicle or 4OHT and passaged once (p1), twice (p2), or six times (p6) after treatment. (C) Immunoblot analysis of ES lines containing the GT and KO alleles at the indicated passage, or a Kdm1a FL/FL Cre-ERT ES line treated with vehicle (Veh) or 4OHT after two passages. (D) Relative DNMT1 levels from C were quantified using ImageJ software. (E) Kdm1a Fl/Fl ES cells were transfected with Cre recombinase. Seventy hours after transfection, cells were immunostained with the indicated antibodies and overlaid with DAPI. (F) Bisulfite sequencing analysis of the indicated repetitive elements was performed on Kdm1a FL/FL Cre-ERT ES cells treated with vehicle or 4OHT and passaged two (p2) or six (p6) times.